SARS-CoV-2変異株 組み換え抗原|東京未来スタイルは研究用試薬の輸入商社です。

背景重要な変異株製品一覧分析データ

背景

複数のSARS-CoV-2変異種が世界的に流行しています。いくつかの変異種は2020年の秋に出現しました。
特筆すべき例：
イギリスのSARS-CoV-2変異株(20B/501Y.V1またはVOC 202012/01またはB.1.1.7 系統として知られています)が異常に多くの突然変異を伴って出現しました。この変異体はその後、アメリカやカナダを含む世界中の多くの国で検出されています。
南アフリカでは、SARS-CoV-2の別の変異株(20C/501Y.V2またはB.1.351 系統として知られています)がB.1.1.7系統とは独立して出現しました。
この変異株はB.1.1.7系統と共通する突然変異をいくつか有しています。この変異株に起因する症例は、南アフリカ以外でも検出されています。
>>> SARS-CoV-2の侵入経路

科学者たちはこれらの変異株の詳細を学び、これらがどれほど簡単に伝染するか、そして現在認可されているワクチンが人々を変異株から守れるのかどうかを一層理解するために取り組んでいます。
ACROBiosystemsはこれらの変異株の組み換え抗原コレクションを開発することに全力で取り組んでいます。
このページで紹介する試薬は、抗体およびワクチン接種の有効性を評価するために使用できます。

特徴

重要な変異が網羅されている	本物の変異株に似せた造り	特別設計の構造
• K417N E484K N501Y D614G……	• 複数個所変異したフルサイズのスパイクタンパク質3量体	• 融合前の立体配座(prefusion conformation)を6P変異により安定化 • S1/S2フーリン開裂部位を2A変異により無効化 • 従来の性質を維持

重要な変異株

イギリス: B.1.1.7南アフリカ: B.1.351ブラジル: P.1
カリフォルニア: B.1.427/B.1.429その他変異株

製品一覧

Critical VariantsShow All

Cat.No.	Species	Product description
SPN-C52H6	B.1.1.7	SARS-CoV-2 (COVID-19) S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag
SPN-C82E5	B.1.1.7	Biotinylated SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His,Avitag™
SPN-C52H3	B.1.1.7/B.1.351/P.1	SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)
SPN-C82E3	B.1.1.7/B.1.351/P.1	Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)
SPN-C52Hc	B.1.351	SARS-CoV-2 S protein (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V), His Tag
SPN-C52Hk	B.1.351	SARS-CoV-2 (COVID-19) S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V), His Tag
SPN-C82E4	B.1.351	Biotinylated SARS-CoV-2 S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V), His,Avitag™
SPN-C52Hg	P.1	SARS-CoV-2 (COVID-19) S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), His Tag
SPN-C82E6	P.1	Biotinylated SARS-CoV-2 S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), His,Avitag™
SPN-C52Hd		SARS-CoV-2 S protein (F817P, A892P, A899P, A942P, K986P, V987P), His Tag
S1N-C52Hd	B.1.1.7	SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), His Tag
S1N-C52Hc	B.1.1.7	SARS-CoV-2 (COVID-19) S1 protein (Y144del), His Tag
S1N-C52Hf		SARS-CoV-2 (COVID-19) S1 protein (E583D), His Tag
S1N-C52Hn	B.1.351/P.1	SARS-CoV-2 S1 protein (E484K, D614G), His Tag
S1N-C52Hr	B.1.1.7	SARS-CoV-2 (COVID-19) S1 protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H), His Tag
S1N-C52Hp	P.1	SARS-CoV-2 (COVID-19) S1 protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), His Tag
S1D-C52H8		SARS-CoV-2 (COVID-19) S1 protein NTD (A222V), His Tag
S1D-C52H7		SARS-CoV-2 (COVID-19) S1 protein NTD (N234Q), His Tag
S1D-C52H5		SARS-CoV-2 (COVID-19) S1 protein NTD (A262S), His Tag
S1D-C52H4		SARS-CoV-2 (COVID-19) S1 protein NTD (P272L), His Tag
S1D-C52H9	B.1.351	SARS-CoV-2 S1 protein NTD (L18F, D80A, D215G, R246I), His Tag
S1D-C52Hd	B.1.1.7	SARS-CoV-2 (COVID-19) S1 protein NTD (HV69-70del, Y144del), His Tag
S1D-C52Hc	B.1.351	SARS-CoV-2 (COVID-19) S protein NTD (L18F, D80A, D215G, 242-244del, R246I), His Tag
S1D-C52He	P.1	SARS-CoV-2 (COVID-19) S1 protein NTD (L18F, T20N, P26S, D138Y, R190S), His Tag
SPD-S52H5		SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag
SPD-S52H4		SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag
SPD-S52H8		SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag
SPD-C52Hs	B.1.351	SARS-CoV-2 (COVID-19) S protein RBD (K417N), His Tag
SPD-C52Ht	P.1	SARS-CoV-2 (COVID-19) S protein RBD (K417T), His Tag
SPD-S52H7		SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag
SPD-C52Hg		SARS-CoV-2 (COVID-19) S protein RBD (N439K), His Tag
SRD-C52H2		SARS-CoV-2 (COVID-19) S protein RBD (N440K), His Tag
SPD-C52He		SARS-CoV-2 (COVID-19) S protein RBD (L452R), His Tag
SPD-C52Hk		SARS-CoV-2 (COVID-19) S protein RBD (Y453F), His Tag
SPD-C52Hd		SARS-CoV-2 (COVID-19) S protein RBD (A475V), His Tag
SPD-C52H4		SARS-CoV-2 (COVID-19) S protein RBD (G476S), His Tag
SPD-C52Hm		SARS-CoV-2 (COVID-19) S protein RBD (S477N), His Tag
SPD-C52H5		SARS-CoV-2 (COVID-19) S protein RBD (V483A), His Tag
SRD-C52H3	B.1.351	SARS-CoV-2 (COVID-19) S protein RBD (E484K), His Tag
SPD-C52Hf		SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag
SPD-C82E6	B.1.1.7	Biotinylated SARS-CoV-2 S protein RBD (N501Y), His,Avitag™
SPD-C52Hn	B.1.1.7/B.1.351	SARS-CoV-2 (COVID-19) S protein RBD (N501Y), His Tag
SPD-C5253	B.1.1.7	SARS-CoV-2 S protein RBD (N501Y), Fc Tag
SPD-C52Hp	B.1.351	SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag
SPD-C5256	B.1.351	SARS-CoV-2 S protein RBD (K417N,E484K,N501Y), Fc Tag
SPD-C82E5	B.1.351	Biotinylated SARS-CoV-2 S protein RBD (K417N,E484K,N501Y), His,Avitag™
SPD-C52Hr	P.1	SARS-CoV-2 (COVID-19) S protein RBD (K417T, E484K, N501Y), His Tag
SPD-C82E7	P.1	Biotinylated SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His,Avitag™
SPD-C5258	P.1.	SARS-CoV-2 S protein RBD (K417T,E484K,N501Y), Fc Tag
S1N-C52Hg	B.1.1.7/B.1.351/P.1	SARS-CoV-2 (COVID-19) S1 protein (N501Y), His Tag
S1N-C52H9		SARS-CoV-2 (COVID-19) S1 protein (A570D), His Tag
S1N-C5256	B.1.1.7/B.1.351/P.1	SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag
S1N-C82E3	B.1.1.7/B.1.351/P.1	Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitag™
S1N-C52Hb	B.1.1.7	SARS-CoV-2 (COVID-19) S1 protein (P681H), His Tag
S1D-C5253	P.1.	SARS-CoV-2 S1 protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), Fc Tag
S1N-C52Hm	B.1.351	SARS-CoV-2 S1 protein (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G), His Tag
S1D-C5256	B.1.351	SARS-CoV-2 S1 protein (L18F, D80A, D215G, LAL242-244del, R246I, K417N, E484K, N501Y, D614G), Fc Tag
S1N-C52Hk	B.1.1.7	SARS-CoV-2 (COVID-19) S1 protein (HV69-70del, N501Y, D614G), His Tag
S1D-C5254	B.1.1.7	SARS-CoV-2 S1 protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H), Fc Tag
S2N-C52Hc	B.1.351	SARS-CoV-2 (COVID-19) S2 protein (A701V), His Tag
S2N-C52Hd	B.1.1.7	SARS-CoV-2 (COVID-19) S2 protein (T716I, S982A, D1118H), His Tag
S2N-C52He	P.1	SARS-CoV-2 (COVID-19) S2 protein (T1027I, V1176F), His Tag
SPD-C82E3	B.1.427/B.1.429	Biotinylated SARS-CoV-2 S protein RBD (L452R), His,Avitag™
S1N-C52Hs	B.1.427/B.1.429	SARS-CoV-2 S1 protein (W152C, L452R, D614G), His Tag
NUN-C52Hm		SARS-CoV-2 Nucleocapsid protein (P13L), His Tag
NUN-C52Hc	P.1	SARS-CoV-2 Nucleocapsid protein (P80R), His Tag
NUN-C52H5		SARS-CoV-2 Nucleocapsid protein (D103Y), His Tag
NUN-C52He		SARS-CoV-2 Nucleocapsid protein (S194L), His Tag
NUN-C52H6		SARS-CoV-2 Nucleocapsid protein (S197L), His Tag
NUN-C52Hd	B.1.351	SARS-CoV-2 Nucleocapsid protein (T205I), His Tag
NUN-C52H3		SARS-CoV-2 Nucleocapsid protein (S202N), His Tag
NUN-C52H9		SARS-CoV-2 Nucleocapsid protein (L230F), His Tag
NUN-C52Hf		SARS-CoV-2 Nucleocapsid protein (I292T), His Tag
NUN-C52H4		SARS-CoV-2 Nucleocapsid protein (Q382H), His Tag
NUN-C52Hg		SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag
NUN-C52H8	B.1.1.7	SARS-CoV-2 Nucleocapsid protein (D3L, R203K, G204R, S235F), His Tag

分析データ

MALS分析により純度の高さが示されています。

His Tag付きのSARS-CoV-2スパイクタンパク質(カタログ番号SPN-C52H6)の純度は90％以上でした。
SEC-MALS分析した結果、タンパク質の分子量は520-560 kDaでした。

ACE2や中和抗体への高い結合性

固定化したHis Tag付きSARS-CoV-2のS1タンパク質(D614G変異株)(カタログ番号S1N-C5256)を1ウェル100 ulのウェルプレートに2 μg/ml の濃度となるよう撒くと、ヒトのACE2やFc Tag（カタログ番号AC2-H5257)と結合します。グラフは0.2-3 ng/mlの範囲で直線を描きます。	固定化したHis Tag付きSARS-CoV-2のS1タンパク質(D614G変異株)（カタログ番号S1N-C5256)を1ウェル100 ulのウェルプレートに2 μg/ml の濃度となるよう撒くと、Anti-SARS-CoV-2中和抗体やヒトIgG1(カタログ番号SAD-S35)と結合します。グラフは0.2-3 ng/mlの範囲で直線を描きます。